Leica DM IRB Epifluorescence Microscope
Inverted epifluorescence microscope for fixed and fluorescently labeled samples. Located in Selby Hall.
Overview
The Leica DM IRB is an inverted epifluorescence microscope equipped with the Leica DFC700T digital camera. It offers multiple filter sets for visualizing common fluorescent dyes and proteins, making it ideal for fixed and live-cell fluorescence imaging.
Key Features
- Inverted epifluorescence microscope with Leica DFC700T digital camera
- Multiple filter sets covering UV, GFP/FITC, and rhodamine channels
- Compatible with slides, dishes, and multi-well plates
- Suitable for both fixed and live-cell imaging
Common Applications
- Fluorescence imaging of fixed cells and tissue sections
- Live-cell imaging for short-term observation
- Co-localization studies using multiple fluorophores
- General fluorescence screening before confocal imaging
Available Filter Sets
| Filter | Excitation/Emission | Common Use |
|---|---|---|
| UV | Chroma A: BP 340-380 / 400 / LP425 | DAPI |
| Broadband FITC/GFP | Chroma I3: BP450-490 / 510 / LP515 | GFP, FITC |
| Narrow band FITC/GFP | Chroma +L5: 480/40 / 505 / BP527/30 | GFP, FITC |
| Rhodamine | Chroma N2.1: BP515-560 / 580 / LP590 | Rhodamine, TRITC |
Filter Sets
Technical Information
| Component | Details |
|---|---|
| Microscope | Leica DM IRB inverted epifluorescence microscope |
| Camera | Leica DFC700T digital camera |
| Objectives | 5x - 100x |
| Location | Selby Hall, Room 006 |
Access and Support
The Leica DM IRB is available for independent use after training by RASC personnel. To schedule training or discuss your project, contact RASC Staff.
Contact RASC Staff