Leica S6D Stereo Microscope
A stereo dissecting microscope with fluorescence capability. Located in Selby Hall.
Overview
The Leica S6D is a versatile stereo dissecting microscope equipped with the NightSea fluorescence system, enabling fluorescence imaging on whole specimens without any modification to the microscope. Ideal for dissection, genotype screening, and fluorescence visualization of transgenic organisms, whole organs, and large tissue samples.
Key Features
- NightSea fluorescence system with multiple excitation/emission combinations
- MC170HD digital camera for image capture
- No sectioning required — image intact specimens directly
- Easy switching between fluorescence wavelength sets
- Compatible with a wide range of fluorescent proteins and fluorophores
Common Applications
- Screening of transgenic organisms — Drosophila, zebrafish, C. elegans
- Genotype sorting and fluorescence-aided dissection
- Whole-mount fluorescence imaging of plant and animal tissue
- Pre-screening samples before confocal or electron microscopy
Available Wavelength Sets
| Code | Excitation | Emission | Common Fluorophores |
|---|---|---|---|
| UV | 360–380 nm | 415 nm LP | DAPI |
| VI | 400–415 nm | 450 nm LP | CFP |
| RB | 440–460 nm | 500 nm LP | GFP, eGFP, Fluorescein |
| CY | 490–515 nm | 550 nm LP | YFP, Venus |
| GR | 510–540 nm | 600 nm LP | DsRed, dTomato |
Technical Information
| Component | Details |
|---|---|
| Microscope | Leica S6D stereo dissecting microscope |
| Fluorescence | NightSea fluorescence illumination system |
| Camera | Leica MC170HD digital camera |
| Objectives | 10x |
| Location | Selby Hall |
Access and Support
Access and Support The Leica S6D is available for independent use after training by RASC personnel. To schedule training or discuss your project Contact RASC Staff
Contact RASC Staff