Capillary Sequencing

Applied Biosystems SeqStudio Genetic Analyzer is a low-throughput benchtop system that delivers Sanger sequencing and fragment analysis. This is a reliable, easy-to-use instrument with an all-in-one cartridge with a single polymer for sequencing and fragment analysis applications.

Sanger sequencing uses the 3'-BigDye dideoxynucleotide triphosphates labeling chemistry. It requires a DNA template, a sequencing primer, a thermostable DNA polymerase, nucleotides (dNTPs), fluorescently labeled 3'-BigDye dideoxynucleotides (ddNTPs), and buffer. The components are combined in a reaction that is subjected to cycles of annealing, extension, and denaturation in a thermal cycler. Thermal cycling of the sequencing reactions creates and amplifies extension products that are terminated by one of the four dideoxynucleotides. The ratio of deoxynucleotides to dideoxynucleotides is optimized to produce a balanced population of long and short extension products.